Difference gel electrophoresis : methods and protocols

Summary: The second edition of this volume provides a comprehensive update of this key method on gel-based proteomics. Chapters present an introduction into the development of methods on principles of differential protein labeling and two-dimensional gel electrophoresis, techniques on optimized proteomic workflows using advanced mass spectrometry for protein identification, and the application of those methods in basic biological research, pathobiology and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Record details

ISBN: 9781071628300 (hardcover)
Physical Description: print
xii, 485 pages : charts, illustrations (some color) ; 26 cm
Edition: Second edition.
Publisher: New York, NY : Humana Press, [2023]
Copyright: ©2023

Content descriptions

Bibliography, etc. Note:

Includes bibliographical references and index.

Formatted Contents Note:

Part I. Introduction. Two-Dimensional Gel Electrophoresis and 2D-DIGE / Paula Meleady -- Part II. Difference gel electrophoresis. Top-down Proteomics and Comparative 2D-DIGE Analysis / Kay Ohlendieck -- DIGE Analysis Software and Protein Identification Approaches / Paul Dowling -- Native DIGE for Quantitative and Functional Analysis of Protein Interactomes / Diksha Dani [and 1 other] -- Comparative Two-dimensional Fluorescence Gel Electrophoresis / Doreen Ackermanm [and 1 other] -- Identification of Ubiquitination associated Proteins using 2D-DIGE / Paul Dowling [and 1 other] -- DIGE-based Phosphoproteomic Analysis / Taras Stasyk [and 1 other] -- DIGE-based Biomarker Discovery in Blood Cancers / Katie Dunphy [and 1 other] -- DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-fixed Paraffin-embedded (FFPE) Tissue / Paul Dowling -- DIGE Analysis of ProteoMiner™ Fractionated Serum/Plasma Samples / Sandra Murphy [and 1 other] -- Comparative 3-sample 2D-DIGE Analysis of Skeletal Muscles / Kay Ohlendieck -- Proteomic identification of saliva proteins as non-invasive diagnostic biomarkers Eleanor M. O'Sullivan [and 3 others] -- Analysis of the saliva proteome using 2D-DIGE / Paul Dowling [and 1 other] -- Part IV. Comparative DIGE tissue proteomics. DIGE Analysis of Clinical Specimens / Cecilia Gelfi [and 1 other] -- DIGE Analysis of Animal Tissues / Alessio Di Luca [and 3 others] -- Rapid 2D-DIGE Proteomic Analysis of Mouse Liver within One Week / Shotaro Kamata [and 1 other] -- 2D-DIGE analysis of liver disease in mice / Aisha Callebaut [and 1 other] -- 2-Dye Versus 3-Dye DIGE for Comparative Testis Tissue Proteomic Analysis / Ashley Holland -- 2-CyDye based 2D-DIGE Analysis of Aged Human Muscle Biopsy Specimens / Kay Ohlendieck -- Identification of Subproteomic Markers for Skeletal Muscle Profiling / Paul Dowling [and 3 others] -- DIGE Analysis of Fish Tissues / Joanna Nynca [and 2 others] -- Part V. Auxiliart DIGE methods. Sample preparation and protein determination for 2D-DIGE proteomics / Stephen Gargan and Kay Ohlendieck -- Protein Digestion for 2D-DIGE Analysis / Sandra Murphy and Kay Ohlendieck -- Subcellular Fractionation for DIGE-based Proteomics / Sandra Murphy -- DIGE Analysis of Immunodepleted Plasma / Paul Dowling and Kay Ohlendieck -- Bioinformatic Analysis of the Subproteomic Profile of Cardiomyopathic Tissue / Sandra Murphy [and 3 others] -- Part VI. DIGE verification analysis. Elucidating Cellular Metabolism and Protein Difference Data from DIGE Proteomics Experiments using Enzyme Assays / Andrew Dowd -- Enzyme Assay Methods to Validate DIGE Proteomics Data / Andrew Dowd -- Immunoblot Analysis of DIGE-based Proteomics / Martin Landsberger [and 1 other] -- Verification of Protein Changes determined by 2D-DIGE based Proteomics using Immunofluorescence Microscopy / Margit Zweyer [and 2 others] -- Histological and Histochemical Microscopy used to verify 2D-DIGE Pathoproteomics / Margit Zweyer [and 2 others] -- Index.